Nanoscopic medicine: the next frontier.

The extraordinary progress that has taken place in cell science and optical nanoscale microscopy has led recently to the concept of medical nanoscopy. Here, we lay out a concept for developing live cell nanoscopy into a comprehensive diagnostic and therapeutic scheme referred to as nanoscopic medicine, which integrates live cell nanoscopy with the structural and functional studies of nanoscopic protein machines (NPMs), the systems biology of NPMs, fluorescent labeling, nanoscopic analysis, and nanoscopic intervention, in order to advance the medical frontier toward the nanoscopic fundament of the cell. It aims at the diagnosis and therapy of diseases by directly visualizing, analyzing, and modifying NPMs and their networks in living cells and tissues.